DNase I (RNase-free )

Deoxyribonuclease I (DNase I) is an endonuclease that degrades doubleand single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ cuts randomly double strand DNA at any site, DNase I with Mn2+ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.

Concentration: 3 units/μl Storage At -20˚C for two years. Shipping Dry ice (-70℃).

Categorie: Molecular biology reagents

Réactifs pour biologie Moléculaire

Sous-catégorie : cDNA synthesis

cDNA synthesis
Fiche Technique